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Immunofluorescence tissues were mounted with ProLong Gold (Life Technologies, Cat# P36934), while H&E and immunohistochemistry tissues were mounted with Permount mounting media (Fisher Scientific, Cat# SP15500) and coverslipped for imaging

GHK-Cu lowers NFkB, TNF-alpha, and Interleukin 6, the cytokines that trigger the inflammatory response

The reason

Step 1: Exposure to UV radiation generates Reactive Oxygen Species (ROS) [3] Step 2: High levels of ROS induce oxidative stress, which leads to Tyrosinase activation, the enzyme responsible for melanin synthesis

Protocols generally run for 12 to 26 weeks, with metabolic labs monitored at baseline, 8 weeks, and periodically thereafter